Biosynthesis of Silver Nanoparticles using Juglans Regia Green Husk (Walnut) Water Extract and Evaluation Antibacterial Activity

Zahra Izadiyan; Kamyar Shameli; Zahra Maghareh Esfahan; Siti Husnaa Mohd Taib; Pooneh Kia

doi:10.37934/jrnn.3.1.90102

Authors

Zahra Izadiyan Department of Environment and Green Technology, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, 54100 Kuala Lumpur, Malaysia
Kamyar Shameli Department of Environment and Green Technology, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, 54100 Kuala Lumpur, Malaysia
Zahra Maghareh Esfahan Department of Chemical & Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia
Siti Husnaa Mohd Taib Department of Environment and Green Technology, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, 54100 Kuala Lumpur, Malaysia
Pooneh Kia Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

DOI:

https://doi.org/10.37934/jrnn.3.1.90102

Keywords:

Silver nanoparticles, biosynthesis, Juglan sregia, green husk, antimicrobial activity

Abstract

Biosynthesis of Ag-NPs at room temperature by using Juglans regia (J. regia) green husk extract which acts as reductant and stabilizer, simultaneously. The Ag/J. regia were characterized by using UV–visible, zeta potential, TEM, and AFM. Formation of Ag/ J. regia was determined by UV–vis spectroscopy, where absorption maxima surface plasmon at 400-460 nm. The zeta potential analysis indicated that J. regia green husk extract was negative and increasing in Ag/ J. regia. TEM images show the mean particle size was 31.37 nm with the standard deviation of 7.1 nm, where confirm by AFM measurements. The XRD study indicates the crystalline nature of the Ag-NPs. The antibacterial activity of Ag-NPs was investigated against Gram-positive and Gram-negative bacteria by the disc diffusion method were found to have high antibacterial activity. These results show that Ag-NPs can be useful in different biologic research and biomedical applications.