Production of Omega-3 Fatty Acids and Astaxanthin from Chlorella vulgaris and Haematococcus pluvialis Cultivated in Chicken Manure Medium

Abstract

Chicken manure contains a high concentration of nitrogen and phosphate, which can be used to replace microalgae commercial medium. Cultivating microalgae by using chicken manure as the nutrients can reduce culture cost. The aims of this research are to study the effect of different concentrations of chicken manure on the growth of algae, Chlorella vulgaris and Haematococcus pluvialis. The concentration of omega-3 fatty acids in C. vulgaris and astaxanthin in H. pluvialis was determined by cultivating C. vulgaris and H. pluvialis in Bold Basal medium and Rudic`s medium respectively and compared with chicken manure medium (CM). The highest concentration of omega-3 fatty acid was obtained in C. vulgaris 14.8% in 10% of CM due to depletion of nitrogen which causes the increase of fatty acid production in the microalgae. In H. pluvialis, 50% of CM gave the highest concentration of astaxanthin produced (0.082 mg/mL) in H. pluvialis due to low concentration of phosphorus in the medium for astaxanthin production. In comparison to nitrate, phosphorus concentration had a more significant impact on astaxanthin synthesis in H. pluvialis. In this research, effective culture of C. vulgaris and H. pluvialis were shown using nutrients i.e., CM instead of commercial medium to obtain the valuable products.