Prone-to-Infectivity of Omicron BA.2 Subvariant from Indonesia on ACE-2 Expressing Cell Lines

Authors

  • Wahyu Hidayati Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia
  • Desti Hidayati Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta 10320, Indonesia
  • Fathur Luthfiano Khairindra Infectious Disease and Immunology Cluster, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia
  • Munawir Umakappa Magister Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia
  • Pratiwi Pudjilestari Sudarmono Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia
  • Siti Farida Department of Medical Pharmacy, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia
  • I Gede Made Wirabrata Agency for Health Policies Development, Indonesian Ministry of Health, Jakarta 10560, Indonesia
  • Beti Ernawati Dewi Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia

DOI:

https://doi.org/10.37934/armne.21.1.5465

Keywords:

ACE-2, COVID-19, Indonesia, Omicron, SARS-CoV-2

Abstract

SARS-CoV-2 virus undergoes mutation, leading to the virus's evolution and modifications in the characteristics of the virus. Omicron, including BA.2 subvariant, is currently the predominant variant in SARS-CoV-2 infection. There are no reports regarding its properties or the utilization of BA.2 Indonesian isolate for therapy and vaccine development. Therefore, this study evaluated appropriate host cells for Omicron BA.2 Indonesian isolate via susceptibility tests. The Omicron BA.2 from Indonesia was exposed to three mammalian-ACE2-expressing cell lines. Sharing amino acids between BA.2 from Indonesia and previous VOC Omicron subvariants was performed using a simple in silico comparison method. The results showed that the virus could not infect HepG2 and Huh-7D12 due to no foci forming on those cell lines. Moreover, we also found that BA.2 Indonesian isolate has a unique amino acid alteration on spike protein. According to the findings, the Omicron BA.2 from Indonesia could propagate on Vero E6 cell lines, and the mutations could play a role in the virus's changing infection mechanism. A deeper in vitro and in silico experiment could enhance the findings by comparing all BA.2 sequences from Indonesia and analyzing the infection mechanism by each single mutation using pseudovirus.

Author Biographies

Wahyu Hidayati, Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia

wahyu_hidayati@uhamka.ac.id

Desti Hidayati, Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta 10320, Indonesia

hidayati_desti@gmail.com

Fathur Luthfiano Khairindra, Infectious Disease and Immunology Cluster, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia

fathrandroe@gmail.com

Munawir Umakappa, Magister Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia

elfatihmunex@gmail.com

Pratiwi Pudjilestari Sudarmono, Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia

psdrmn@yahoo.com

Beti Ernawati Dewi, Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia

beti.ernawati@ui.ac.id

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Published

2024-07-31

How to Cite

Hidayati, Wahyu, Desti Hidayati, Fathur Luthfiano Khairindra, Munawir Umakappa, Pratiwi Pudjilestari Sudarmono, Siti Farida, I Gede Made Wirabrata, and Beti Ernawati Dewi. 2024. “Prone-to-Infectivity of Omicron BA.2 Subvariant from Indonesia on ACE-2 Expressing Cell Lines”. Journal of Advanced Research in Micro and Nano Engineering 21 (1):54-65. https://doi.org/10.37934/armne.21.1.5465.
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