Optimization of NPK Medium with the Addition of Mung Bean Sprout Extract for Biomass and Lipid Production of Stanieria HS-48 as a Potential Biofuel

Abstract

Stanieria strain HS-48 from Ciater hot spring, Indonesia contains lipid content in the form of fatty acids that can be used as a raw material for biodiesel. Optimization of the growth medium was used to produce high contents of microalgal lipids. The growth medium in this study utilized NPK medium with the addition of variations in the concentration of mung bean sprout extract 2% and 4%. This study aimed to determine the best concentration of mung bean sprout extract in NPK medium for the highest biomass and lipid production of Stanieria HS-48. The experiments were conducted in airlift photobioreactors (APBR) for 19 days. The highest total cell density of Stanieria HS-48 in NPK medium with the addition of variations in the concentration of mung bean sprout extract 2% and 4% were 6.165 x 10⁶ and 4.702 x 10⁶ cell/ml. The lipid content of the biomass from Stanieria HS-48 on NPK medium with the addition of mung bean sprout extract 2% and 4% were 62.9% and 44.8%. The study showed that the NPK medium with the addition of the concentration of mung bean sprout extract 2% was a good growth medium for increasing biomass and lipid of Stanieria HS-48.